Regulatory
M5 TNN

Part:BBa_K202002:Design

Designed by: Poonam Srivastava   Group: iGEM09_Minnesota   (2009-10-16)


Hybrid promoter having multiple operator sites.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 192
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The regulatory architecture is designed such that each operator's position efficiently interferes with RNA polymerase (RNAp) promoter binding without inhibiting promoter function.


Source

synthesized using tetO2 (mutation at position 5) and lacO1.

References